派洛宁Y对环氧树脂半薄切片的正色和异染染色反应。

Orthochromatic and metachromatic staining reactions by pyronin Y on Epon semithin sections.

作者信息

Armas-Portela R, Gutierrez-Gonzalvez M G, Stockert J C

出版信息

Acta Histochem. 1984;74(1):1-4. doi: 10.1016/S0065-1281(84)80016-1.

PMID:6203318

Abstract

Semithin sections from glutaraldehyde-fixed, Epon-embedded tissues were stained by aqueous solutions of pyronin Y at increasing concentrations (from 10(-6) to 10(-3) mol). Mucopolysaccharide containing structures (e.g. mucin) were found stained in orange, meanwhile the chromatin and remaining tissue components appeared in a bright pink-red color. Cytophotometric measurements showed that a metachromatic shift occurs in the mucin content from goblet cells after pyronin Y staining at 10(-3) mol. Some features of the metachromatic reactions by cationic dyes are briefly discussed.

摘要

将戊二醛固定、环氧树脂包埋的组织制成半薄切片，用浓度递增（从10⁻⁶到10⁻³摩尔）的派洛宁Y水溶液进行染色。发现含有粘多糖的结构（如粘蛋白）被染成橙色，同时染色质和其余组织成分呈现出亮粉红色。细胞光度测量显示，在10⁻³摩尔的派洛宁Y染色后，杯状细胞的粘蛋白含量发生了异染性变化。简要讨论了阳离子染料异染反应的一些特点。