环磷酸腺苷对肾ATP酶活性的调节作用。
Modulation of renal ATPase activities by cyclic AMP.
作者信息
Giesen E M, Imbs J L, Grima M, Schmidt M, Schwartz J
出版信息
Biochem Biophys Res Commun. 1984 Apr 30;120(2):619-24. doi: 10.1016/0006-291x(84)91300-7.
A possible link between cellular cyclic AMP content and Na+K+ATPase activity was investigated in homogenates of rat kidney. Enzyme kinetics of Mg2+ and Na+K+ATPase were run in the presence of cyclic AMP, dibutyryl cAMP and compounds expected to elevate cyclic AMP levels such as forskolin, a potent adenylate cyclase activator, IBMX, an inhibitor of phosphodiesterases, and the beta-agonist isoproterenol. Medullary Na+K+ATPase is strongly inhibited by cyclic AMP whereas cortical Na+K+ATPase was stimulated in the same conditions. The correlation between ATPase activity and cellular cyclic AMP content supports the concept of a possible regulation of the enzyme by cyclic AMP.
在大鼠肾脏匀浆中研究了细胞内环磷酸腺苷（cAMP）含量与钠钾ATP酶（Na⁺K⁺ATPase）活性之间的可能联系。在环磷酸腺苷、二丁酰环磷腺苷（dibutyryl cAMP）以及预期能提高环磷酸腺苷水平的化合物存在的情况下，进行了镁离子和钠钾ATP酶的酶动力学实验，这些化合物包括强效腺苷酸环化酶激活剂福斯可林（forskolin）、磷酸二酯酶抑制剂异丁基甲基黄嘌呤（IBMX）以及β-激动剂异丙肾上腺素。髓质钠钾ATP酶受到环磷酸腺苷的强烈抑制，而在相同条件下皮质钠钾ATP酶则受到刺激。ATP酶活性与细胞内环磷酸腺苷含量之间的相关性支持了环磷酸腺苷可能对该酶进行调节的概念。
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