在终末分化期间，通过肌球蛋白重链cDNA探针在肌肉细胞培养物中检测到的一种瞬时存在的聚腺苷酸加尾（poly(A)+）RNA物种。
A transient species of poly(A)+ RNA detected by a myosin heavy chain cDNA probe in muscle cell culture during terminal differentiation.
作者信息
Liang R
出版信息
Biochem Biophys Res Commun. 1984 May 16;120(3):741-6. doi: 10.1016/s0006-291x(84)80169-2.
PMID:6203526
Abstract
Primary cell cultures were prepared from breast muscles of 11 day 4 hour-embryonic chicks. Cytoplasmic RNAs were isolated from the cultured cells at various time intervals from day 3 to day 8. A [P32] DNA probe complementary to messenger RNA of myosin heavy chain was used to hybridize with the RNAs after gel electrophoresis. A transient species of polyadenylated RNA with a decreased mobility in electrophoresis was detected during a period of time when contractions of syncytial fibers were first observed.
摘要
原代细胞培养物取自11日龄4小时胚胎期雏鸡的胸肌。在培养的细胞中，于第3天至第8天的不同时间间隔分离细胞质RNA。在凝胶电泳后，使用与肌球蛋白重链信使RNA互补的[P32]DNA探针与RNA杂交。在首次观察到多核纤维收缩的一段时间内，检测到一种电泳迁移率降低的瞬时多聚腺苷酸化RNA。
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