Chow D A, Miller V E, Carlson G A, Pohajdak B, Greenberg A H
Invasion Metastasis. 1981;1(4):205-19.
The contribution of natural killer (NK) cells to natural antitumor resistance (NR) in syngeneic DBA/2 mice was examined. Subclones from two NK-resistant tumors, P815 and L5178Y, were selected for tumorigenicity in NR assays measuring the tumor frequency of threshold doses and the elimination of 131IUdR-labelled cells. In vivo variation in sensitivity to NR did not correlate with in vitro NK cytolysis for these clones, but did for a third pair of clones from the SL2 lymphoma selected on the basis of sensitivity or resistance to NK lysis. In another series of experiments it was found that a decrease in NK cytolysis of the (NK-sensitive) SL2-5 lymphoma in aged DBA/2 contrasted with an increased rate of elimination and lower tumor frequency, suggesting that the ontogeny of these phenomena was not related. In addition, while interferon treatment of the (NK-sensitive) SL2-5 produced a corresponding decrease in susceptibility to NK cytolysis in vitro and a reduction in the in vivo elimination of 131I-labelled cells, tumor of the NK-resistant phenotype, interferon-treated in the same way, remained NK-resistant and was more rapidly eliminated. The failure to observe correlative changes in NK cytolysis associated with heterogeneity in tumorigenicity and with age-related or interferon-induced changes in NR suggest that NR cannot be due solely to an NK effector mechanism.
研究了自然杀伤（NK）细胞对同基因DBA/2小鼠天然抗肿瘤抗性（NR）的贡献。从两种NK抗性肿瘤P815和L5178Y中挑选亚克隆，用于NR测定中的致瘤性检测，该测定测量阈剂量的肿瘤频率以及131IUdR标记细胞的清除情况。对于这些克隆，体内对NR敏感性的变化与体外NK细胞溶解作用不相关，但对于基于对NK裂解的敏感性或抗性从SL2淋巴瘤中挑选出的第三对克隆而言，二者相关。在另一系列实验中发现，老年DBA/2小鼠中（NK敏感的）SL2 - 5淋巴瘤的NK细胞溶解作用降低，而清除率增加且肿瘤频率降低，这表明这些现象的发生发展并无关联。此外，虽然对（NK敏感的）SL2 - 5进行干扰素处理后，其体外对NK细胞溶解作用的敏感性相应降低，且体内131I标记细胞的清除减少，但以同样方式处理的NK抗性表型肿瘤仍具有NK抗性且清除更快。未观察到与致瘤性异质性以及与年龄相关或干扰素诱导的NR变化相关的NK细胞溶解的相关变化，这表明NR不能仅归因于NK效应机制。
