Chow D A
J Natl Cancer Inst. 1984 Feb;72(2):339-46.
The contribution of natural antibodies (NAb) to the selective elimination of tumor variants in vivo was studied to further support the hypothesis that NAb participate in host-mediated natural resistance (NR) against neoplasia. Tumors obtained after the sc, ip, or iv inoculation of the natural killer cell-resistant, natural antibody-sensitive L5178Y-F9 tumor clone into syngeneic DBA/2 mice were tested for their sensitivity to NR in the [131I]5-iodo-2'-deoxyuridine elimination assay and to serum NAb in the presence of complement. Cells removed from the ip or sc sites of tumor inoculation exhibited a reduced susceptibility to syngeneic NR and to syngeneic but not to allogeneic NAb. Tumors recovered from the spleen, brain, and lungs after iv inoculation also expressed a decreased sensitivity to both syngeneic NR and NAb. These data suggest that NR against tumors is widely disseminated and support the hypothesis that NAb participate in this antitumor defense, including the control of metastatic spread of disease. Tumors previously selected in vivo also exhibited reduced sensitivity to complement-mediated lysis by NAb acquired in the peritoneum. The correlation observed between this sensitivity to complement and the recovery of tumor cells after the ip injection of L5178Y-F9 contribute to the evidence that NAb acts in vivo.
研究了天然抗体（NAb）在体内对肿瘤变体的选择性清除作用，以进一步支持NAb参与宿主介导的针对肿瘤的天然抵抗力（NR）这一假说。将对自然杀伤细胞有抗性、对天然抗体敏感的L5178Y-F9肿瘤克隆经皮下、腹腔内或静脉内接种到同基因的DBA/2小鼠后获得的肿瘤，在[131I]5-碘-2'-脱氧尿苷清除试验中检测其对NR的敏感性，并在有补体存在的情况下检测其对血清NAb的敏感性。从肿瘤接种的腹腔或皮下部位取出的细胞，对同基因NR和同基因但非异基因NAb的敏感性降低。静脉内接种后从脾脏、脑和肺中回收的肿瘤，对同基因NR和NAb的敏感性也降低。这些数据表明，针对肿瘤的NR广泛分布，并支持NAb参与这种抗肿瘤防御，包括控制疾病的转移扩散这一假说。先前在体内选择的肿瘤，对腹膜中获得的NAb介导的补体溶解也表现出较低的敏感性。在腹腔注射L5178Y-F9后，观察到这种对补体的敏感性与肿瘤细胞的回收之间的相关性，为NAb在体内起作用提供了证据。
