Toguchi T, Burmester G, Nunez-Roldan A, Gregersen P, Seremetis S, Lee S, Szer I, Winchester R
Hum Immunol. 1984 Jun;10(2):69-81. doi: 10.1016/0198-8859(84)90074-0.
The monoclonal antibodies 109d6 and IVD12 reacted with separate polymorphic Ia epitopes in immunofluorescent studies. Using a panel of lymphoblastoid B cell lines, antibody 109d6 reacted with all HLA-DR4 or DR7 positive lines in a pattern resembling the MT3 specificity recognized by human alloantisera. The antibody IVD12 reacted with all HLA-DR4 and two of three DR5 positive B cell lines suggesting that it recognized a specificity similar to MB3. The intensity of fluorescence was greater on DR5(+) cell lines than on DR4(+) cell lines relative to the amount of a nonpolymorphic Ia determinant. Among 45 unrelated control individuals reactivity with antibody 109d6 was correlated most closely with (r = 0.724) but not identical in occurrence to the MT3 specificity. Cocapping experiments demonstrated that the 109d6 epitope and the IVD12 epitope were present on independently redistributed cell surface molecules of DR4 homozygous lymphoblastoid cell lines. Furthermore, the DR4 alloantigens detected by an absorbed polyclonal human alloserum were similarly identified on molecules that were independent from those bearing either the 109d6 epitope or the IVD12 epitope. Taken together, these data indicate the existence of at least four distinct, serologically defined Ia molecular species.
在免疫荧光研究中，单克隆抗体109d6和IVD12与不同的多态性Ia表位发生反应。使用一组淋巴母细胞样B细胞系，抗体109d6与所有HLA - DR4或DR7阳性细胞系发生反应，其反应模式类似于人同种抗血清识别的MT3特异性。抗体IVD12与所有HLA - DR4以及三个DR5阳性B细胞系中的两个发生反应，这表明它识别的特异性类似于MB3。相对于非多态性Ia决定簇的量，DR5(+)细胞系上的荧光强度比DR4(+)细胞系上的更强。在45名无关对照个体中，与抗体109d6的反应性与MT3特异性的相关性最为密切（r = 0.724），但在出现情况上并不完全相同。共帽实验表明，109d6表位和IVD12表位存在于DR4纯合淋巴母细胞样细胞系独立重新分布的细胞表面分子上。此外，通过吸收的多克隆人同种血清检测到的DR4同种抗原在与携带109d6表位或IVD12表位的分子独立的分子上也被类似地鉴定出来。综上所述，这些数据表明至少存在四种不同的、血清学定义的Ia分子种类。
