Stepanik T M, Sottrup-Jensen L
J Biol Chem. 1984 Jul 10;259(13):8310-2.
The amino acid sequences have been determined for three CNBr fragments of human alpha 2-macroglobulin which form a disulfide-bridged Mr = 40,000 fragment set. The fragments are located in the COOH-terminal part of alpha 2-macroglobulin (CB18, residues 776-936; CB23, residues 1292-1355; and CB26, residues 1376-1451). CB18 contains one glucosamine-based carbohydrate group attached to Asn-71 and two internal disulfide bridges (Cys-23 bound to Cys-51 and Cys-49 bound to Cys-85, respectively). Cys-123 is disulfide bridged to Cys-7 of CB23. Cys-38 of CB23 is disulfide bridged to Cys-69 of CB26. CB26, which contains one glucosamine-based carbohydrate group attached to Asn-26, is the COOH-terminal CNBr fragment. CB18, CB23, and CB26 account for 301 of the 1451 residues of the subunit of alpha 2-macroglobulin.
已确定人α2-巨球蛋白的三个CNBr片段的氨基酸序列，这些片段形成了一个由二硫键连接的Mr = 40,000片段组。这些片段位于α2-巨球蛋白的COOH末端部分（CB18，第776 - 936位残基；CB23，第1292 - 1355位残基；以及CB26，第1376 - 1451位残基）。CB18含有一个连接到Asn-71的基于葡糖胺的碳水化合物基团和两个内部二硫键（Cys-23分别与Cys-51以及Cys-49与Cys-85形成二硫键）。Cys-123与CB23的Cys-7形成二硫键。CB23的Cys-38与CB26的Cys-69形成二硫键。CB26是COOH末端的CNBr片段，含有一个连接到Asn-26的基于葡糖胺的碳水化合物基团。CB18、CB23和CB26占α2-巨球蛋白亚基1451个残基中的301个。
