Frol'kis V V, Tanin S A, Martsinko V I, Kul'chitskiĭ O K
Neirofiziologiia. 1984;16(2):189-94.
Aqueous solution of L-leucine-14C (specific activity 339 mCi/mmol) was introduced in a dose of 7-8 microliter to the ventral horn (L5,6) of the spinal cord of adult (8-12 month) and old (26-28 month) rats. The radioactivity of various parts of the corresponding ventral roots was measured after 1-2.5 hrs. Labelled substances (including protein) were found to migrate with fast flow in adult rats with the rate of 408 +/- 10.9 and 380 +/- 22 mm/24 hrs, respectively, as compared with 217 +/- 11.3 and 200 +/- 40 mm/24 hrs in old rats. The axoplasmic flow slows down in old rats with the increase of distance from the neuronal body. Uncoupling of oxidation and phosphorylation due to intraperitoneal administration of 2.4-dinitrophenol, NaF inhibition of glycolysis, hypoxic hypoxia produce more marked deceleration of axoplasmatic flow in old rats, while small doses of NaF accelerate the flow, which correlates with the rise of cAMP level in the ventral roots.
将L-亮氨酸-14C水溶液（比活度为339 mCi/mmol）以7 - 8微升的剂量注入成年（8 - 12个月）和老年（26 - 28个月）大鼠脊髓腹角（L5、6）。1 - 2.5小时后测量相应腹根各部分的放射性。结果发现，标记物质（包括蛋白质）在成年大鼠中以快速流动迁移，速率分别为408±10.9和380±22毫米/24小时，而在老年大鼠中为217±11.3和200±40毫米/24小时。老年大鼠的轴浆流随着与神经元体距离的增加而减慢。腹腔注射2,4 - 二硝基苯酚导致氧化与磷酸化解偶联、氟化钠抑制糖酵解、低氧性缺氧在老年大鼠中使轴浆流更明显地减慢，而小剂量的氟化钠则加速轴浆流，这与腹根中cAMP水平的升高相关。
