5-氮杂胞苷核苷酸的化学合成及3'-末端含5-氮杂胞苷的tRNA的制备。
Chemical synthesis of 5-azacytidine nucleotides and preparation of tRNAs containing 5-azacytidine in its 3'-terminus.
作者信息
Zielinski W S, Sprinzl M
出版信息
Nucleic Acids Res. 1984 Jun 25;12(12):5025-36. doi: 10.1093/nar/12.12.5025.
Abstract
5-azacytidine-5'-triphosphate prepared from 5-azacytidine by chemical phosphorylation is a substrate for AMP (CMP) tRNA nucleotidyl transferase from yeast. tRNAsPhe from yeast containing 5-azacytidine in their 3'-termini were prepared enzymatically. tRNAPhe-Cpn5CpA and tRNAPhe-n5Cpn5CpA can be aminoacylated by phenylalanyl-tRNA synthetase from yeast and they are active in the poly(U)-dependent synthesis of poly(Phe) on E. coli ribosomes. The decomposition of 5-azacytidine via hydrolysis of the triazine ring is significantly accelerated by a phosphate group on the 5'-position of the nucleotide. After the incorporation of 5-azacytidine-5'-phosphate into a polynucleotide chain the rate of hydrolysis of the triazine ring decreases considerably.
摘要
通过化学磷酸化由5-氮杂胞苷制备的5-氮杂胞苷-5'-三磷酸是酵母中AMP（CMP）tRNA核苷酸转移酶的底物。通过酶法制备了3'-末端含有5-氮杂胞苷的酵母tRNAsPhe。tRNAPhe-Cpn5CpA和tRNAPhe-n5Cpn5CpA可以被酵母苯丙氨酰-tRNA合成酶氨酰化，并且它们在大肠杆菌核糖体上的聚（U）依赖性聚（Phe）合成中具有活性。核苷酸5'-位上的磷酸基团显著加速了5-氮杂胞苷通过三嗪环水解的分解。在将5-氮杂胞苷-5'-磷酸掺入多核苷酸链后，三嗪环的水解速率大大降低。
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