Daubeze M, Narbonne J F
Toxicology. 1984 Jun;31(3-4):315-8. doi: 10.1016/0300-483x(84)90112-4.
2,4,5,2',4',5'-[14C] Hexachlorobiphenyl (2,4,5-HCB) a slowly metabolized PCB was given to rats by gastric incubation. The hepatocyte nuclei (HCN) were then isolated and treated with specific hydrolytic enzymes in order to separate the nucleic macromolecules, protein RNA and DNA. The results show that 2,4,5-HCB binds in vivo to hepatocyte nuclei. Liver nuclear proteins bind 70% of 2,4,5-HCB and 30% is found in the DNA fraction. No radioactivity was found in the nuclear RNA fraction at this experimental time (16 h).
2,4,5,2',4',5'-[¹⁴C]六氯联苯（2,4,5-HCB）是一种代谢缓慢的多氯联苯，通过胃内灌喂给予大鼠。然后分离出肝细胞核（HCN），并用特定的水解酶进行处理，以分离核酸大分子、蛋白质、RNA和DNA。结果表明，2,4,5-HCB在体内与肝细胞核结合。肝核蛋白结合了70%的2,4,5-HCB，30%存在于DNA部分。在这个实验时间（16小时），核RNA部分未发现放射性。
