用于双水相系统的蛋白质吸附剂。
Protein adsorbents intended for use in aqueous two-phase systems.
作者信息
Hedman P O, Gustafsson J G
出版信息
Anal Biochem. 1984 May 1;138(2):411-5. doi: 10.1016/0003-2697(84)90830-3.
PMID:6204552
Abstract
Preparation of adsorbents with high partition coefficients in polyethylene glycol-dextran and polyethylene glycol-phosphate systems is described. These adsorbents may be used to carry to carry proteins away from the insoluble cell fragments generated during cell disruption. By chromatographic elution, proteins may be selectively desorbed in a reduced volume.
摘要
本文描述了在聚乙二醇-葡聚糖和聚乙二醇-磷酸盐体系中制备具有高分配系数的吸附剂的方法。这些吸附剂可用于从细胞破碎过程中产生的不溶性细胞碎片中分离蛋白质。通过色谱洗脱，蛋白质可以在较小体积中被选择性地解吸。
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