乙醇对碱性磷酸酶同工酶差异沉淀的进一步观察

Further observations on the differential precipitation of alkaline phosphatase isoenzymes by ethanol.

作者信息

Samuelson R C, Moss D W

出版信息

Clin Chim Acta. 1978 Feb 1;83(1-2):167-70. doi: 10.1016/0009-8981(78)90220-6.

PMID:620461

Abstract

The proportions of the total activities of different isoenzymes of human alkaline phosphatase precipitated from serum by ethanol (20% v/v) were: liver phosphatase, 37%; placental phosphatase, 23%; bone phosphatase, 8.0%, and small-intestinal phosphatase, 3.7%. Treatment of the isoenzymes with neuraminidase reduced the percentages of non-intestinal phosphatases precipitated by ethanol to below 10%. Precipitation of intestinal alkaline phosphatase was unaffected by this treatment. The degree of solubility in ethanol therefore appears to be largely determined by the content of terminal sialic acid residues in the alkaline phosphatase molecules. In contrast the stabilities of the isoenzymes to heating at 56 degrees C were not significantly altered by neuraminidase digestion.

摘要

用乙醇（20% v/v）从血清中沉淀出的人碱性磷酸酶不同同工酶的总活性比例如下：肝磷酸酶为37%；胎盘磷酸酶为23%；骨磷酸酶为8.0%，小肠磷酸酶为3.7%。用神经氨酸酶处理这些同工酶后，乙醇沉淀的非肠道磷酸酶百分比降至10%以下。这种处理对肠道碱性磷酸酶的沉淀没有影响。因此，在乙醇中的溶解度似乎很大程度上由碱性磷酸酶分子中末端唾液酸残基的含量决定。相比之下，神经氨酸酶消化对同工酶在56℃加热的稳定性没有显著改变。