Loblay R H, Pritchard-Briscoe H, Basten A
Aust J Exp Biol Med Sci. 1984 Feb;62 ( Pt 1):11-25. doi: 10.1038/icb.1984.2.
Regulation of the antibody response to the hapten, dinitrophenyl (DNP), was studied using human gammaglobulin (HGG) as the carrier in an adoptive transfer system. CBA mice immunized at least 4 weeks previously to HGG or DNP served as the source of T helper cells and hapten-primed B cells, respectively. The addition of spleen cells from donors recently primed to HGG in immunogenic form (aHGG) suppressed the collaborative anti-DNP PFC response as effectively as cells from tolerant donors. The suppressive effect was antigen-specific and was mediated by a T cell with the same phenotype (Ly-1-, Ly-23+, Ia+) and induction kinetics as those previously identified in HGG-tolerant animals. During the primary response to HGG an early burst of helper activity was detected initially, followed by a wave of suppression which peaked at day 7 and subsequently waned, allowing adoptive helper function to reappear during the third week. When previously immunized animals were boosted with soluble HGG after primary suppression had waned, the sequential appearance of helper and suppressor activity was accelerated, and the secondary suppressive effect was more profound and of longer duration than that observed during a primary response. Although helper activity was not apparent during the peak of secondary suppression, helper T cells (Th) had not been functionally deleted since treatment of the donor spleen cells with anti-Ia and complement to deplete suppressor T cells (Ts) before adoptive transfer resulted in significant augmentation of the anti-DNP response. The secondary suppressive effect was formally shown to be mediated by activated Ts effector cells bearing the same surface markers as the cells responsible for primary suppression. The results suggest that the Ts population, like other lymphocyte subsets, contain memory cells capable of rapid reexpression of effector function upon secondary exposure to antigen. These cells are considered to be of a major importance in maintenance of immune homeostasis.
在过继转移系统中，以人丙种球蛋白（HGG）作为载体，研究了对半抗原二硝基苯基（DNP）抗体应答的调节。分别以至少在4周前用HGG或DNP免疫的CBA小鼠作为T辅助细胞和半抗原致敏B细胞的来源。添加来自最近以免疫原形式用HGG致敏的供体（aHGG）的脾细胞，与来自耐受供体的细胞一样有效地抑制了协同抗DNP PFC应答。抑制作用是抗原特异性的，由具有与先前在HGG耐受动物中鉴定的相同表型（Ly-1-、Ly-23+、Ia+）和诱导动力学的T细胞介导。在对HGG的初次应答期间，最初检测到早期的辅助活性爆发，随后是一波抑制，在第7天达到峰值，随后减弱，使得过继辅助功能在第三周重新出现。当初次抑制减弱后，用可溶性HGG对先前免疫的动物进行加强免疫时，辅助和抑制活性的顺序出现加速，并且二次抑制作用比初次应答期间观察到的更深刻且持续时间更长。尽管在二次抑制峰值期间辅助活性不明显，但辅助性T细胞（Th）并未发生功能缺失，因为在过继转移前用抗Ia和补体处理供体脾细胞以耗尽抑制性T细胞（Ts）导致抗DNP应答显著增强。正式证明二次抑制作用是由具有与负责初次抑制的细胞相同表面标志物的活化Ts效应细胞介导的。结果表明，Ts群体与其他淋巴细胞亚群一样，包含记忆细胞，这些记忆细胞在再次接触抗原时能够快速重新表达效应功能。这些细胞被认为在维持免疫稳态中至关重要。
