Chart H, Trust T J
Can J Microbiol. 1984 May;30(5):703-10. doi: 10.1139/m84-105.
The technique of immunoblotting was used to identify the surface antigens of the marine vibrios pathogenic for fish, Vibrio anguillarum and Vibrio ordalii. Polyclonal antisera raised in rabbits to strains representing the two most common serotypes causing Vibriosis in fish in North America were used. The results demonstrated that antigenic specificity was conferred by the lipopolysaccharides, with three serotypes being displayed among the strains examined. The lipopolysaccharides of strains chosen as type species for V. anguillarum and V. ordalii displayed antigenic cross-reactivity. The morphological heterogeneity of the Vibrio lipopolysaccharides was also analyzed in silver-stained polyacrylamide gels and by intrinsic 32P-radiolabelling. Two distinct lipopolysaccharide morphologies were exhibited, one with 0 polysaccharide chains of heterogeneous chain length, the other having 0 polysaccharide chains of more uniform chain length but displaying microheterogeneity. These lipopolysaccharide morphologies corresponded to different serogroups. Two minor proteins of apparent molecular weights 49000-51000 present in outer membrane preparations isolated by the sarcosinate extraction procedure were also strong antigens, and common to all strains of V. anguillarum tested and to several strains of V. ordalii. The major outer membrane protein was a weak antigen common to both species.
采用免疫印迹技术鉴定对鱼类致病的海洋弧菌——鳗弧菌和奥氏弧菌的表面抗原。使用在兔体内产生的针对代表北美鱼类弧菌病最常见的两种血清型菌株的多克隆抗血清。结果表明，脂多糖赋予了抗原特异性，在所检测的菌株中显示出三种血清型。被选为鳗弧菌和奥氏弧菌模式种的菌株的脂多糖表现出抗原交叉反应性。还通过银染聚丙烯酰胺凝胶和内在的³²P放射性标记分析了弧菌脂多糖的形态异质性。呈现出两种不同的脂多糖形态，一种具有链长不均一的O多糖链，另一种具有链长更均匀但显示微异质性的O多糖链。这些脂多糖形态对应于不同的血清群。通过肌氨酸提取程序分离的外膜制剂中存在的两种表观分子量为49000 - 51000的次要蛋白质也是强抗原，并且在所测试的所有鳗弧菌菌株和几种奥氏弧菌菌株中都存在。主要外膜蛋白是这两个菌种共有的弱抗原。
