Lieser M, Heinrich P C
Eur J Biochem. 1984 Jun 15;141(3):461-4. doi: 10.1111/j.1432-1033.1984.tb08214.x.
Nuclear RNP complexes, cytoplasmic mRNP particles and free and membrane-bound polysomes were prepared from rat liver and their low-molecular-mass RNA components were analyzed on polyacrylamide/formamide gels. The separated small RNAs transferred to diazophenylthioether paper were hybridized to the nick-translated recombinant plasmid pA6 containing cDNA sequences for the low-Mr RNA called 7S(L) RNA. Nuclear RNP particles and free and membrane-bound polysomes were found to contain 7S(L) RNA. In the cytoplasm 7S(L) RNA could be identified as the major small RNA in 20-S cmRNP particles.
从大鼠肝脏中制备了核核糖核蛋白复合物、细胞质信使核糖核蛋白颗粒以及游离和膜结合多核糖体，并在聚丙烯酰胺/甲酰胺凝胶上分析了它们的低分子量RNA成分。转移到重氮苯硫醚纸上的分离出的小RNA与含有名为7S(L)RNA的低分子量RNA cDNA序列的切口平移重组质粒pA6杂交。发现核核糖核蛋白颗粒以及游离和膜结合多核糖体含有7S(L)RNA。在细胞质中，7S(L)RNA可被鉴定为20-S细胞质信使核糖核蛋白颗粒中的主要小RNA。
