Krell J, Rabin B S
Diabetes. 1984 Aug;33(8):709-11. doi: 10.2337/diab.33.8.709.
Antibody to the islet cells of the pancreas is detected in the serum of many patients with recent onset, insulin-dependent diabetes. Islet cell antibodies have been detected using the procedure of indirect immunofluorescence. We have adopted an immunohistochemical procedure using glucose oxidase to the histochemical identification of islet cell antibody. This procedure was found to be more sensitive than the immunofluorescence procedure, and, in addition, it was much easier to determine when a positive reaction was present. Thus, the glucose-oxidase immunohistochemical procedure for detecting antibodies to islet cells is preferable in comparison with indirect immunofluorescence.
在许多近期发病的胰岛素依赖型糖尿病患者血清中可检测到胰腺胰岛细胞抗体。胰岛细胞抗体已通过间接免疫荧光法检测到。我们采用了一种使用葡萄糖氧化酶的免疫组织化学方法来进行胰岛细胞抗体的组织化学鉴定。发现该方法比免疫荧光法更敏感，此外，确定阳性反应何时出现要容易得多。因此，与间接免疫荧光相比，用于检测胰岛细胞抗体的葡萄糖氧化酶免疫组织化学方法更可取。
