van Toorenenbergen A W, Dieges P H
Int Arch Allergy Appl Immunol. 1984;75(1):44-7. doi: 10.1159/000233588.
Patients A and M developed allergic symptoms when working in a greenhouse with paprika plants and freesia plants, respectively. The possible involvement of an IgE-mediated mechanism was investigated with the skin prick test, radioallergosorbent test (RAST) and the histamine release test (HRT). Paprika flower, leaf and stem extract released 58, 47 and 43% of the total amount of histamine from washed leukocytes of patient A. In serum A IgE antibodies against paprika leaves and flowers could be demonstrated by RAST (11% binding of 125I-anti-IgE added). Freesia flower and stem extract released 46 and 43% histamine, respectively, from washed leukocytes of patient M. In the RAST, specific IgE antibodies against freesia flowers and stems were found in serum M (37% binding of 125I-anti-IgE added).
患者A和患者M分别在种植甜椒植物和小苍兰植物的温室中工作时出现了过敏症状。通过皮肤点刺试验、放射变应原吸附试验（RAST）和组胺释放试验（HRT）研究了IgE介导机制的可能参与情况。甜椒花、叶和茎提取物从患者A洗涤后的白细胞中释放出组胺总量的58%、47%和43%。通过RAST可在血清A中检测到针对甜椒叶和花的IgE抗体（添加的125I-抗IgE结合率为11%）。小苍兰花和茎提取物分别从患者M洗涤后的白细胞中释放出46%和43%的组胺。在RAST中，在血清M中发现了针对小苍兰花和茎的特异性IgE抗体（添加的125I-抗IgE结合率为37%）。
