van Toorenenbergen A W, Waanders J, Gerth Van Wijk R, Vermeulen A M
Department of Clinical Chemistry, University Hospital Rotterdam Dijkzigt, Rotterdam, The Netherlands.
Int Arch Allergy Immunol. 2000 Aug;122(4):246-50. doi: 10.1159/000024405.
The high incidence of occupational allergy in horticulture has only recently been recognized. We determined IgE against pollen and fruit from paprika and tomato plants in sera from 3 greenhouse workers and in 3 sera from food-allergic patients.
Proteins in extracts of paprika and tomato pollen were incubated with patients' sera after covalent coupling of these proteins to agarose beads, or in immunoblots.
IgE against paprika pollen, but no IgE against tomato pollen, was found in serum from 2 greenhouse workers who worked with paprika plants only. IgE binding of these 2 sera to agarose-bound paprika pollen extract could be inhibited by paprika pollen but not by tomato pollen extract. A greenhouse worker, who cultivated tomato plants, had IgE against both tomato and paprika pollen. IgE binding of this serum to agarose-bound paprika pollen extract could be inhibited by both paprika pollen and tomato pollen extract. Three food-allergic patients also had IgE against tomato and paprika pollen. IgE from 2 food-allergic patients recognized IgE-binding structures in paprika or tomato pollen that were also present in fruit from the corresponding plant. In contrast, no substantial cross-reactivity was observed between paprika pollen and fruit towards IgE from 3 greenhouse workers. In 4 of 5 sera that were positive in the paprika pollen immunoblot major IgE binding to allergens of about 30 and 64 kD occurred.
The presence of IgE against paprika or tomato pollen is not restricted to workers in horticulture; IgE against these pollen can also be present in food-allergic patients who have serum IgE against paprika and/or tomato fruit.
园艺业中职业性过敏的高发病率直到最近才被认识到。我们测定了3名温室工人血清以及3名食物过敏患者血清中针对辣椒和番茄植株花粉及果实的IgE。
将辣椒和番茄花粉提取物中的蛋白质与琼脂糖珠共价偶联后，或在免疫印迹中，与患者血清一起孵育。
在仅接触辣椒植株的2名温室工人血清中发现了针对辣椒花粉的IgE，但未发现针对番茄花粉的IgE。这2份血清与琼脂糖结合的辣椒花粉提取物的IgE结合可被辣椒花粉抑制，但不能被番茄花粉提取物抑制。一名种植番茄植株的温室工人同时具有针对番茄和辣椒花粉的IgE。该血清与琼脂糖结合的辣椒花粉提取物的IgE结合可被辣椒花粉和番茄花粉提取物均抑制。3名食物过敏患者也具有针对番茄和辣椒花粉的IgE。2名食物过敏患者的IgE识别辣椒或番茄花粉中也存在于相应植株果实中的IgE结合结构。相比之下，在3名温室工人中，未观察到辣椒花粉与果实之间对IgE的显著交叉反应。在辣椒花粉免疫印迹呈阳性的5份血清中的4份中，主要的IgE与约30和64kD的过敏原结合。
针对辣椒或番茄花粉的IgE不仅存在于园艺工人中；针对这些花粉的IgE也可能存在于对辣椒和/或番茄果实具有血清IgE的食物过敏患者中。
