Ekimoto H, Takada K, Takahashi K, Matsuda A, Takita T, Umezawa H
J Antibiot (Tokyo). 1984 Jun;37(6):659-63. doi: 10.7164/antibiotics.37.659.
The pulmonary fibrosis caused by peplomycin (PEP) was studied in terms of oxygen toxicity using ICR mice. When 16 micrograms of PEP was administered intratracheally in mice after exposure to the air containing 75% O2 for 10 days, the pulmonary fibrosis was completely suppressed, while when mice were exposed to 75% O2 after the administration of PEP, the fibrosis was much severe than that of mice raised in atmospheric air. In 50% O2, similar oxygen effect was also observed, but it was weaker than that in 75% O2. In 90% O2, the oxygen toxicity was observed in mice without administration of PEP. When mice were exposed to 75% O2, the activities of superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase, which are relevant to the detoxication of active oxygen species, were not increased in the lung, but the levels of reducing agents such as glutathione and ascorbic acid, and high molecular substances having 1O2-scavenging activity were enhanced. The results suggest that these materials have some roles to decrease the pulmonary fibrosis caused by PEP.
使用ICR小鼠，从氧毒性方面对培洛霉素（PEP）引起的肺纤维化进行了研究。在暴露于含75%氧气的空气中10天后，向小鼠气管内注射16微克PEP时，肺纤维化被完全抑制，而在注射PEP后将小鼠暴露于75%氧气中时，纤维化比在大气环境中饲养的小鼠严重得多。在50%氧气环境中，也观察到了类似的氧效应，但比在75%氧气环境中弱。在90%氧气环境中，未注射PEP的小鼠也出现了氧毒性。当小鼠暴露于75%氧气中时，与活性氧解毒相关的超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶和谷胱甘肽还原酶的活性在肺中并未增加，但谷胱甘肽和抗坏血酸等还原剂以及具有单线态氧清除活性的高分子物质的水平有所提高。结果表明，这些物质在减轻PEP引起的肺纤维化方面具有一定作用。
