Kitamura K, Nakauchi H, Koyasu S, Yahara I, Okumura K, Tada T
J Immunol. 1984 Sep;133(3):1371-8.
A cloned effector-type suppressor T cell line, 3D10, which is known to suppress the antibody response against dinitrophenylated keyhole limpet hemocyanin (KLH), produced a soluble KLH-specific factor (TsF) that can replace the function of parental T cell clones. High activity of TsF was released spontaneously into the culture supernatant when cultured in interleukin 2 (IL 2)-containing medium, requiring no antigenic stimulation. The culture supernatant of 3D10 was also capable of inhibiting the KLH-induced proliferative response of primed T cells in an antigen-specific manner. The direct target of TsF was found to be Lyt-1+2- T cells undergoing an early stage of antigen-specific proliferation. TsF was antigen binding but lacked any other serologic markers such as I-J and immunoglobulin heavy chain-linked allotypic determinants on T cells. No genetic restriction was found in its action on allogeneic T cells. The production of IL 2 in proliferative T cells by antigenic stimulation was not inhibited by TsF. These results indicate that the TsF described here is the legitimate mediator produced by the effector-type suppressor T cell that suppresses the antigen-specific responses of Lyt-1+2- T cells. The m.w. of TsF was approximately 75,000.
一种克隆的效应型抑制性T细胞系3D10，已知其能抑制针对二硝基苯基化钥孔血蓝蛋白（KLH）的抗体反应，该细胞系产生了一种可溶性的KLH特异性因子（TsF），它可以替代亲代T细胞克隆的功能。当在含白细胞介素2（IL-2）的培养基中培养时，TsF的高活性会自发释放到培养上清液中，无需抗原刺激。3D10的培养上清液也能够以抗原特异性的方式抑制KLH诱导的致敏T细胞的增殖反应。发现TsF的直接靶标是处于抗原特异性增殖早期阶段的Lyt-1+2-T细胞。TsF能结合抗原，但缺乏任何其他血清学标志物，如T细胞上的I-J和免疫球蛋白重链连锁的同种异型决定簇。在其对同种异体T细胞的作用中未发现遗传限制。TsF不抑制抗原刺激在增殖性T细胞中产生IL-2。这些结果表明，这里描述的TsF是由效应型抑制性T细胞产生的、抑制Lyt-1+2-T细胞抗原特异性反应的合法介质。TsF的分子量约为75,000。
