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通过单克隆抗体分析表位密度和抗体亲和力对酶联免疫吸附测定(ELISA)的影响。

The effect of epitope density and antibody affinity on the ELISA as analysed by monoclonal antibodies.

作者信息

Lew A M

出版信息

J Immunol Methods. 1984 Aug 3;72(1):171-6. doi: 10.1016/0022-1759(84)90445-9.

DOI:10.1016/0022-1759(84)90445-9
PMID:6205095
Abstract

The antibody titre and the optical density values in an ELISA are influenced by the epitope density of the antigen and the affinity of the antibody tested. This has major implications in the interpretation of ELISA results.

摘要

酶联免疫吸附测定(ELISA)中的抗体滴度和光密度值受抗原表位密度以及所检测抗体亲和力的影响。这对ELISA结果的解读具有重要意义。

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