Cram D, Ray A, O'Gorman L, Skurray R
Plasmid. 1984 May;11(3):221-33. doi: 10.1016/0147-619x(84)90028-3.
Transcriptional activity associated with the leading region (53.8-66.7F) in F DNA transfer has been shown by RNA-DNA hybridization studies to occur on the anterior segment extending from 59.4 to 66.7F. Promoter-probe analysis of cloned leading region segments detected two promoters within the transcribed portion of the leading region. The promoter active across the 64.7F EcoRI site on the transferred F strand was associated with the expression of two polypeptides, 6d and 13.5p, located between 64.7-66.6F. However, no definite role could be ascribed to the second promoter operative through the 66.6F Bg/II site located in close proximity to oriT, the origin of transfer.
通过RNA-DNA杂交研究表明，F DNA转移过程中与前导区（53.8-66.7F）相关的转录活性发生在从59.4到66.7F延伸的前段。对克隆的前导区片段进行启动子探针分析，在前导区的转录部分检测到两个启动子。在转移的F链上跨越64.7F EcoRI位点具有活性的启动子，与位于64.7-66.6F之间的两种多肽6d和13.5p的表达相关。然而，对于通过紧邻转移起点oriT的66.6F Bg/II位点起作用的第二个启动子，无法确定其明确作用。
