Zurbriggen A, Vandevelde M, Beranek C F, Steck A
Res Vet Sci. 1984 May;36(3):270-5.
Three different regions from neonatal dog brain were mechanically dissociated and cultured. At seven, 10, 14 and 19 days, cultures were harvested and studied with immunocytochemical techniques for the demonstration of astrocytes, oligodendrocytes and fibroblasts. The canine brain cultures were confluent at seven days and contained fibroblasts, both types of glial cells and several small fragments of undissociated brain tissue. Many cells were myelin associated glycoprotein (MAG) positive. In the older cultures increasing numbers of myelin basic protein (MBP) positive oligodendrocytes were also demonstrated. Double labelling studies demonstrated that MBP and MAG were produced by the same cells. MAG positive cells were therefore identified as oligodendrocytes. Most intensive oligodendroglial growth occurred in the vicinity of undissociated tissue fragments. The results were discussed in respect to glial differentiation and the question of whether the canine cultures may contain neurons.
从新生犬脑的三个不同区域进行机械分离并培养。在第7、10、14和19天，收获培养物并用免疫细胞化学技术进行研究，以显示星形胶质细胞、少突胶质细胞和成纤维细胞。犬脑培养物在第7天汇合，包含成纤维细胞、两种类型的神经胶质细胞以及一些未分离的脑组织小碎片。许多细胞髓鞘相关糖蛋白（MAG）呈阳性。在较老的培养物中，也证实了髓鞘碱性蛋白（MBP）阳性的少突胶质细胞数量增加。双重标记研究表明MBP和MAG由相同的细胞产生。因此，MAG阳性细胞被鉴定为少突胶质细胞。最强烈的少突胶质细胞生长发生在未分离的组织碎片附近。就神经胶质细胞分化以及犬类培养物是否可能含有神经元的问题对结果进行了讨论。
