Ramaekers F, Haag D, Jap P, Vooijs P G
Acta Cytol. 1984 Jul-Aug;28(4):385-92.
Antibodies to intermediate filament proteins were used to characterize tumor cells present in peritoneal and pleural effusions and in thin needle aspirates from palpable lymph nodes. Metastatic adenocarcinoma cells (breast, ovary, endometrium, cervix, colon and stomach) as well as squamous-cell carcinomas and mesotheliomas stained specifically with antibodies to keratin while mesenchymally derived tumor cells (lymphomas, melanoma, fibrosarcoma and neurofibrosarcoma) were positive only for vimentin. Especially in cases of lymph node aspirates, keratin staining in cells was a direct indication of metastatic carcinoma. Antibodies to these different components of the cytoskeleton can thus be used in cytopathologic diagnosis when a definitive diagnosis cannot be made on the basis of conventional cytologic features.
应用针对中间丝蛋白的抗体来鉴定存在于腹腔积液、胸腔积液以及可触及淋巴结细针穿刺样本中的肿瘤中的肿瘤细胞。转移性腺癌细胞（乳腺癌、卵巢癌、子宫内膜癌、宫颈癌、结肠癌和胃癌）以及鳞状细胞癌和间皮瘤对角蛋白抗体呈特异性染色，而间充质来源的肿瘤细胞（淋巴瘤、黑色素瘤、纤维肉瘤和神经纤维肉瘤）仅对波形蛋白呈阳性反应。尤其在淋巴结穿刺样本中，细胞角蛋白染色直接提示转移性癌。因此，当基于传统细胞学特征无法做出明确诊断时，针对细胞骨架这些不同成分的抗体可用于细胞病理学诊断。
