Clarke P G
Acta Histochem. 1984;74(2):235-50. doi: 10.1016/S0065-1281(84)80013-6.
In chick embryos, unlike in adults, many neurons can be impregnated selectively with the LOYEZ iron-haematoxylin method, and with the MBSN and ARN types of Luxol Fast Blue. The same neurons are selected with each of the 3 methods. Their distribution at a given age is roughly predictable, but it changes with age, as is described in some detail. Large neurons are impregnated more readily than small neurons; glial cells are not impregnated. With each of the 3 methods, the same conditions of fixation and embedding are needed for the selective impregnation to occur: fixation with a dilute, alkaline solution of formaldehyde (or, non-optimally, a closely related aldehyde), and nitrocellulose embedding. Numerous other stains fail to produce the selective impregnation. The parameters of the LOYEZ method have been varied systematically in order to optimize the neuronal impregnation, and preliminary efforts have been made to deduce the substrate of the staining, which probably involves proteins or peptides.
在鸡胚中，与成年个体不同，许多神经元可用洛耶兹铁苏木精法以及髓鞘碱性蛋白染色法（MBSN）和嗜银网状纤维染色法（ARN）类型的Luxol Fast Blue选择性浸染。这三种方法选择的是相同的神经元。它们在特定年龄的分布大致是可预测的，但会随年龄变化，这将在后面详细描述。大神经元比小神经元更容易被浸染；神经胶质细胞不会被浸染。使用这三种方法中的任何一种，要实现选择性浸染都需要相同的固定和包埋条件：用稀释的碱性甲醛溶液（或者，不太理想的情况下，用一种密切相关的醛）固定，并用硝化纤维素包埋。许多其他染色方法无法产生这种选择性浸染。已系统地改变了洛耶兹方法的参数以优化神经元浸染，并已初步尝试推断染色的底物，其可能涉及蛋白质或肽。
