Millar T J, Chubb I W
Brain Res. 1984 Jul 30;307(1-2):303-9. doi: 10.1016/0006-8993(84)90484-0.
Monoclonal antibodies against substance P were used to study the distribution of the peptide in the retinae of chicks that had been kept in either total darkness or brightly lit conditions. In retinae from light-adapted chicks, substance P was found to be in normal sized amacrine cells and giant cells in the inner nuclear layer, in fibres in sublaminae 1,3-5 in the inner plexiform layer, in cells in the ganglion cell layer which may have been mostly displaced amacrine cells and in a few fibres in the optic nerve fibre layer. There was no apparent concentration of immunoreactive cell bodies in any part of the retina. After dark-adaption of the birds, the immunoreactivity in the retinae was much reduced; there were fewer visible cells in all parts of the retina and there was no reaction in the inner plexiform layer. When birds were taken from the dark and kept in the light for different periods, there was a gradual increase in the number of cells and in the overall immunoreaction. Substance P is thus like several other putative retinal neurotransmitters in that its concentration in the retina is regulated by light/dark. These experiments do not indicate how the changes occur, but they underline the need for a strict control of lighting conditions in experiments with these relatively slowly metabolized neurotransmitters.
抗P物质的单克隆抗体被用于研究肽在饲养于完全黑暗或明亮光照条件下的雏鸡视网膜中的分布。在来自光适应雏鸡的视网膜中，发现P物质存在于内核层的正常大小无长突细胞和巨细胞中、内网状层第1、3 - 5亚层的纤维中、神经节细胞层中可能大多为移位无长突细胞的细胞中以及视神经纤维层的少数纤维中。视网膜任何部位均未出现免疫反应性细胞体的明显聚集。鸟类暗适应后，视网膜中的免疫反应性大大降低；视网膜各部位可见细胞减少，内网状层无反应。当鸟类从黑暗环境转移到光照环境不同时间段后，细胞数量和总体免疫反应逐渐增加。因此，P物质与其他几种假定的视网膜神经递质一样，其在视网膜中的浓度受光/暗调节。这些实验并未表明这些变化是如何发生的，但它们强调了在使用这些代谢相对缓慢的神经递质进行实验时严格控制光照条件的必要性。
