以抗原为固相的酶免疫测定法检测妊娠相关血浆蛋白A（联合免疫酶测定法）
Enzyme immunometric assay for the determination of pregnancy associated plasma protein A (PAPP-A) with the antigen as solid phase (conjoint IEMA).
作者信息
Bersinger N A
出版信息
Experientia. 1984 Sep 15;40(9):1022-4. doi: 10.1007/BF01946490.
PMID:6205894
Abstract
Purified pregnancy associated plasma protein A (PAPP-A) can be effectively bound to polystyrene microtitre plates. This immobilized antigen competes with the added serum PAPP-A of unknown concentration for the limited amount of peroxidase-labeled monospecific anti-PAPP-A antibody incubated simultaneously. The sensitivity is 0.1 WHO unit/ml and non-specific binding is 1.0%.
摘要
纯化的妊娠相关血浆蛋白A（PAPP-A）可有效结合到聚苯乙烯微量滴定板上。这种固定化抗原与加入的未知浓度血清PAPP-A竞争同时孵育的有限量过氧化物酶标记的单特异性抗PAPP-A抗体。灵敏度为0.1国际单位/毫升，非特异性结合率为1.0%。
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