Monier S, Le Marchand-Brustel Y
Mol Cell Endocrinol. 1984 Aug;37(1):109-14. doi: 10.1016/0303-7207(84)90133-3.
The effects of insulin and insulin-like growth factor I (IGF-I) on RNA synthesis and the effect of IGF-I on polypeptide chain initiation have been studied in the isolated mouse soleus muscle. Both peptides after a 3 h incubation enhanced net incorporation of the labelled uridine into RNA to a similar extent (40% increase over basal). The maximally effective concentrations were 66 nM and 100 nM for insulin and IGF-I respectively. Actinomycin D prevented the peptides' effect on RNA synthesis without modifying their effect on protein synthesis. Furthermore IGF-I increased the rate of initiation of polypeptide chains. It is suggested that in muscle, IGF-I and insulin stimulate protein synthesis by a dual mechanism: a rapid effect on the rate of polypeptide chain initiation; a slower effect on RNA synthesis.
在分离的小鼠比目鱼肌中研究了胰岛素和胰岛素样生长因子I（IGF-I）对RNA合成的影响以及IGF-I对多肽链起始的影响。孵育3小时后，两种肽都以相似的程度增强了标记尿苷向RNA中的净掺入（比基础水平增加40%）。胰岛素和IGF-I的最大有效浓度分别为66 nM和100 nM。放线菌素D可阻止这些肽对RNA合成的作用，而不改变它们对蛋白质合成的作用。此外，IGF-I提高了多肽链起始的速率。提示在肌肉中，IGF-I和胰岛素通过双重机制刺激蛋白质合成：对多肽链起始速率的快速作用；对RNA合成的较慢作用。
