Koike M, Komori A
Gan. 1984 Jun;75(6):525-33.
A human cancer cell line (ZK-1) has been established from a well-differentiated squamous cell carcinoma of the tongue. The cytokeratin polypeptides pattern of ZK-1 cells consists of four major polypeptides with molecular weights between 46 and 58 kilodaltons (kd). Antibodies raised against the purified 58 kd cytokeratin filament from cultured ZK-1 cells were shown to be specific by one- and two-dimensional polyacrylamide gel electrophoresis, immunofluorescence, and immunoelectron microscopy. Immunoprecipitation studies showed that the antibody reacted mainly with the 58 kd cytokeratin. The distribution of the 58 kd cytokeratin in both sparse and confluent cultures was analyzed by the indirect immunofluorescence technique. In both cases, it appeared that fibrillar arrays extended throughout the cytoplasm running over the nucleus and toward cell-to-cell boundaries. Thick bundles of filaments were seen in confluent cultures, especially in large and flattened keratinized cells. Characteristically, reactions were also seen in the intercellular boundaries, appearing as "dots." Electron microscopy using immunoperoxidase techniques indicated that the 58 kd cytokeratin was localized in tonofilaments, tonofilaments attached to desmosomes, desmosomal plaques and membrane-coating granules.
一种人类癌细胞系（ZK - 1）已从舌部高分化鳞状细胞癌中建立。ZK - 1细胞的细胞角蛋白多肽模式由四种主要多肽组成，分子量在46至58千道尔顿（kd）之间。通过一维和二维聚丙烯酰胺凝胶电泳、免疫荧光和免疫电子显微镜显示，针对从培养的ZK - 1细胞中纯化的58 kd细胞角蛋白丝产生的抗体具有特异性。免疫沉淀研究表明，该抗体主要与58 kd细胞角蛋白反应。采用间接免疫荧光技术分析了58 kd细胞角蛋白在稀疏培养和汇合培养中的分布。在这两种情况下，似乎纤维状排列贯穿整个细胞质，覆盖细胞核并延伸至细胞间边界。在汇合培养物中可见粗的丝束，尤其是在大的扁平角质化细胞中。其特征还在于在细胞间边界也可见反应，呈“点状”。使用免疫过氧化物酶技术的电子显微镜显示，58 kd细胞角蛋白定位于张力丝、附着于桥粒的张力丝、桥粒斑和膜被颗粒中。
