Taniyama T, Watanabe T
Hybridoma. 1983;2(2):161-8. doi: 10.1089/hyb.1983.2.161.
A monoclonal antibody of the IgG2a isotype directed against lymphokine-activated human macrophage cell line (U937) was produced and characterized. By indirect immunofluorescence microscopy or binding assay, this antibody (MaG-1) reacted with human peripheral monocytes, peritoneal macrophages, alveolar macrophages, and peripheral granulocytes but not with lymphocytes, erythrocytes, and platelets. MaG-1 antigen was also expressed by 34% of bone-marrow cells which includes monocytic and granulocytic precursors, whereas lymphoid and erythroid precursor cells were found on MaG-1 negative population. Immunoprecipitation of 125I-labeled U937 cell extract with the Mag-1 antibody under nonreducing and reducing conditions yielded a specific band of 66 kD. Thus, this antibody defines a new human macrophage/monocyte and granulocyte-specific antigen and may be useful for studying differentiation of human macrophages/monocytes.
制备并鉴定了一种针对淋巴因子激活的人巨噬细胞系（U937）的IgG2a同种型单克隆抗体。通过间接免疫荧光显微镜检查或结合试验，该抗体（MaG-1）与人外周血单核细胞、腹腔巨噬细胞、肺泡巨噬细胞和外周粒细胞发生反应，但与淋巴细胞、红细胞和血小板不发生反应。MaG-1抗原也在34%的骨髓细胞中表达，这些骨髓细胞包括单核细胞和粒细胞前体，而淋巴样和红系前体细胞存在于MaG-1阴性群体中。在非还原和还原条件下，用Mag-1抗体对125I标记的U937细胞提取物进行免疫沉淀，产生了一条66 kD的特异性条带。因此，该抗体定义了一种新的人巨噬细胞/单核细胞和粒细胞特异性抗原，可能有助于研究人巨噬细胞/单核细胞的分化。
