Miles J W, Mount D L, Beckmann T J, Carrigan S K, Galoux I M, Hitos P, Hodge M C, Kissler K, Martijn A, Sanchez-Rasero F
J Assoc Off Anal Chem. 1984 Jul-Aug;67(4):834-7.
Although the gas chromatographic separation of the isomers of BHC was demonstrated two decades ago, the present AOAC method of analysis of BHC for gamma-isomer (lindane) content is based on a separation carried out on a liquid chromatographic partition column. A method of analysis has been developed that uses an OV-210 column for separation of the gamma-isomer from the other isomers and impurities in technical BHC. Di-n-propyl phthalate was chosen as an internal standard. The same system allows quantitation of lindane in lotion and shampoo after these products are extracted with ethyl acetate-isooctane (1 + 4). The analytical methods were subjected to a collaborative trial with 10 laboratories. The coefficient of variation for technical BHC was 2.83%. For the water-dispersible powder and emulsifiable concentrate, the coefficients of variation were 2.89% and 4.62%, respectively. Coefficients of variation for 1% lindane lotion and shampoo were 4.36% and 11.92%, respectively. The method has been adopted official first action.
尽管早在二十年前就已证明可用气相色谱法分离六六六的异构体，但目前美国官方分析化学师协会（AOAC）测定六六六γ异构体（林丹）含量的方法是基于在液相色谱分配柱上进行的分离。现已开发出一种分析方法，该方法使用OV - 210柱从工业用六六六中分离γ异构体及其他异构体和杂质。选用邻苯二甲酸二正丙酯作为内标。在用乙酸乙酯 - 异辛烷（1 + 4）萃取乳液和洗发水中的林丹后，同一系统可对其进行定量分析。该分析方法在10个实验室进行了协同试验。工业用六六六的变异系数为2.83%。对于水分散性粉剂和乳油，变异系数分别为2.89%和4.62%。1%林丹乳液和洗发水的变异系数分别为4.36%和11.92%。该方法已被官方首次采用。
