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Identification of several forms of the glial fibrillary acidic protein, or alpha-albumin, by a specific monoclonal antibody.

作者信息

Gheuens J, de Schutter E, Noppe M, Lowenthal A

出版信息

J Neurochem. 1984 Oct;43(4):964-70. doi: 10.1111/j.1471-4159.1984.tb12831.x.

DOI:10.1111/j.1471-4159.1984.tb12831.x
PMID:6206204
Abstract

A monoclonal antibody (mAb), termed UIA/NEU/I/G1 (G1), that reacted with the astroglial marker glial fibrillary acidic protein (GFAP), or alpha-albumin, is described. It was directed against a structural determinant of GFAP. The G1 mAb could be used for quantitative determination of GFAP in two-site radiometric assays and for histoimmunological demonstration of GFAP. The G1 mAb reacted with the GFAP from rat as well as from man. The presence of several different molecular weight forms of GFAP in aqueous and detergent extracts from human brain was shown with the G1 mAb. The possible meaning of these forms is discussed.

摘要

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