Herpers M J, Ramaekers F C, Aldeweireldt J, Moesker O, Slooff J
Acta Neuropathol. 1986;70(3-4):333-9. doi: 10.1007/BF00686093.
The expression of intermediate filament (IF) proteins was studied in 71 cases of malignant human astrocytoma and in 17 cases of reactive gliosis, using immunocytochemical techniques with polyclonal and monoclonal antibodies to glial fibrillary acidic protein (GFAP) and vimentin. In all cases of astrocytoma, varying in degree of malignancy from grade I to grade IV, co-expression of GFAP and vimentin was found. No change in vimentin- or GFAP-IF expression with increasing anaplasia was seen. In addition astrocytic cells in reactive gliosis showed simultaneous expression of GFAP and vimentin. The intracellular distribution of these IF proteins differed. Vimentin was found to be located in a more juxta-nuclear position, whereas GFAP immunoreactivity showed a more intense staining of the cellular processes. Astrocytes in reactive gliosis behaved more or less like neoplastic cells. However, thin cell processes of reactive astrocytes in the cortex and superficial white matter only contained GFAP immunoreactivity. Simultaneous expression of GFAP and vimentin and their proportion in malignant and reactive glial cells are discussed in the light of earlier reports on the IF content of glial cells during development and maturation, in which vimentin precedes GFAP-expression. The existence of two separate (functional) IF systems in astroglia is suggested.
采用针对胶质纤维酸性蛋白（GFAP）和波形蛋白的多克隆及单克隆抗体免疫细胞化学技术，对71例人类恶性星形细胞瘤和17例反应性胶质增生病例中的中间丝（IF）蛋白表达进行了研究。在所有恶性程度从I级到IV级不等的星形细胞瘤病例中，均发现了GFAP和波形蛋白的共表达。随着间变程度增加，波形蛋白或GFAP中间丝表达未见变化。此外，反应性胶质增生中的星形细胞也显示出GFAP和波形蛋白的同时表达。这些中间丝蛋白的细胞内分布有所不同。发现波形蛋白位于更靠近细胞核的位置，而GFAP免疫反应性在细胞突起处染色更强。反应性胶质增生中的星形细胞表现得或多或少类似于肿瘤细胞。然而，皮质和浅表白质中反应性星形细胞的细细胞突起仅含有GFAP免疫反应性。根据先前关于神经胶质细胞在发育和成熟过程中中间丝含量的报道，讨论了GFAP和波形蛋白在恶性和反应性神经胶质细胞中的同时表达及其比例，其中波形蛋白先于GFAP表达。提示星形胶质细胞中存在两个独立的（功能性）中间丝系统。
