Seelig L L, Head J R
Stain Technol. 1984 Jan;59(1):43-7. doi: 10.3109/10520298409113827.
Autoradiographic tracing using tritium labeled compounds or cells is a common laboratory technique for light and electron microscopy. This report describes a chemographic effect associated with certain cells in sections from tissues embedded in the new methacrylate plastic embedding compounds. When tissue sections from rats and rhesus monkeys that received no radioisotope were coated with nuclear track emulsion and subsequently developed, cells with morphologic characteristics of mast cells showed significant grain formation over the entire cell. Three different types of methacrylate plastics were tested using rat and monkey tissues and all three were found to promote grain formation over mast cells; however, this phenomenon was not seen in similar tissue sections from paraffin or epoxy embedded material. The properties of methacrylate plastics which promote positive chemography by mast cells may reflect the greater permeability of this class of plastics. Due to their wide tissue distribution, the presence of such chemographically active cells could cause false estimates of the distribution of either exogenous radiolabeled cells or radioisotopes within many tissues.
使用氚标记化合物或细胞进行放射自显影追踪是光镜和电镜检查中常用的实验室技术。本报告描述了一种与新甲基丙烯酸酯塑料包埋化合物包埋的组织切片中的某些细胞相关的化学显影效应。当未接受放射性同位素的大鼠和恒河猴的组织切片涂上核径迹乳剂并随后显影时，具有肥大细胞形态特征的细胞在整个细胞上显示出明显的颗粒形成。使用大鼠和猴组织测试了三种不同类型的甲基丙烯酸酯塑料，发现所有三种都能促进肥大细胞上的颗粒形成；然而，在石蜡或环氧树脂包埋材料的类似组织切片中未观察到这种现象。甲基丙烯酸酯塑料促进肥大细胞产生阳性化学显影的特性可能反映了这类塑料具有更高的渗透性。由于它们在组织中分布广泛，这类化学活性细胞的存在可能会导致对许多组织中外源性放射性标记细胞或放射性同位素分布的错误估计。
