Rodionov A V
Tsitologiia. 1984 May;26(5):537-42.
Chromosomes of Gallus domesticus were stained with rivanolum-SO2 (fluorescence Feulgen reaction) and by Hoechst 33258. Fluorescence photography was performed on a 35 mm film KN-3(KN-3) "Svema". The negatives were analyzed with the microdensitometer. The Feulgen (Fr--) and the Hoechst 3358 (H--) densitometric profiles of chromosomes showed light and dark segments along the metaphase chromosomes. The amount of DNA, as determined by the fluorescence Feulgen reaction, is not constant along the chromosome arms. Consequently, the base composition is not the only factor influencing the fluorescence of Hoechst 33258 along the chromosomes. The comparative analysis of densitometric profiles of the Hoechst 33258 and rivanolum-SO2 stained chromosomes shows that the Iq telomere band consists of GC-rich DNA (about 70% GC). The variation of DNA contents along the metaphase chromosome arms can be realized at both chromoneme and/or subchromoneme levels.
家鸡的染色体用利凡诺-SO₂（荧光福尔根反应）和Hoechst 33258进行染色。在35毫米胶片KN-3（KN-3）“斯韦马”上进行荧光摄影。用显微密度计分析底片。染色体的福尔根（Fr--）和Hoechst 3358（H--）密度测量图谱显示中期染色体上有亮段和暗段。通过荧光福尔根反应测定的DNA量在染色体臂上并不恒定。因此，碱基组成不是影响Hoechst 33258沿染色体荧光的唯一因素。对Hoechst 33258和利凡诺-SO₂染色染色体的密度测量图谱的比较分析表明，Iq端粒带由富含GC的DNA组成（约70%的GC）。中期染色体臂上DNA含量的变化可以在染色线和/或亚染色线水平上实现。
