Lindgren P G, Lundqvist M, Norheim J, Wilander E, Oberg K
Am J Surg. 1984 Sep;148(3):353-6. doi: 10.1016/0002-9610(84)90470-7.
With the aid of ultrasonography, representative percutaneous biopsy specimens were obtained from 20 of 21 patients (95 percent) with liver metastases of carcinoids and endocrine pancreatic tumors. The specimens were examined with silver stains and immunocytochemically after the application of monoclonal serotonin antibodies. The Grimelius argyrophil silver nitrate stain was positive in all tumor metastases, demonstrating that they were of neurohormonal endocrine type. The argentaffin reaction stained 14 of 15 metastases of small intestinal carcinoids, whereas tumors with other primary sites were unreactive. Immunocytochemical analysis with monoclonal serotonin antibodies stained all metastases of small intestinal carcinoids, and the other endocrine tumor metastases were unreactive. With immunocytochemical analysis, optimal results were obtained in Bouin-fixed tumor specimens, whereas for the argentaffin reaction, formalin was preferable. The results show that silver stains and immunocytochemical analysis with monoclonal serotonin antibodies on small percutaneous biopsy specimens of liver metastases of endocrine tumors and carcinoids are valid for the prediction of the location of the primary tumors.
借助超声检查，从21例类癌和内分泌胰腺肿瘤肝转移患者中的20例（95%）获取了代表性经皮活检标本。标本在应用单克隆血清素抗体后进行银染色和免疫细胞化学检查。Grimelius嗜银硝酸银染色在所有肿瘤转移灶中均呈阳性，表明它们属于神经激素内分泌型。嗜银反应在15例小肠类癌转移灶中的14例呈阳性，而其他原发部位的肿瘤无反应。用单克隆血清素抗体进行免疫细胞化学分析时，所有小肠类癌转移灶均呈阳性，而其他内分泌肿瘤转移灶无反应。在免疫细胞化学分析中，Bouin固定的肿瘤标本能获得最佳结果，而对于嗜银反应，福尔马林固定更佳。结果表明，对内分泌肿瘤和类癌肝转移的经皮小活检标本进行银染色和用单克隆血清素抗体进行免疫细胞化学分析，对于预测原发肿瘤的位置是有效的。
