Wilander E, Lundqvist M, el-Salhy M
J Pathol. 1985 Mar;145(3):251-8. doi: 10.1002/path.1711450306.
A series of 60 fore-gut carcinoid tumours was examined with regard to serotonin content after application of three different techniques, namely: the argentaffin reaction, formalin-induced fluorescence according to Falck-Hillarp and immunocytochemistry with monoclonal antibodies to serotonin. To evaluate the staining-fluorescence of individual tumour cells, the methods were applied to identical tumour sections. Twelve tumours demonstrated serotonin-immunoreactive cells, six of which were also argentaffin. Four tumours contained argentaffin cells but no serotonin-immunoreactivity. With the use of all three techniques, three types of tumour cells occurred, namely: serotonin-immunoreactive, non-argentaffin and non-fluorescence cells, serotonin-immunoreactive, argentaffin and fluorescent cells, and non-serotonin immunoreactive, argentaffin and non-fluorescent cells. The first (serotonin-immunoreactive) cell type was most frequently found in the tumours. One gastric carcinoid in which the argentaffin cells exceeded the serotonin-immunoreactive cells, a positive reaction was found with the modified Warthin-Starry reaction for demonstrating melanin. Since none of the techniques used for visualization of serotonin in endocrine tumours is unquestionably specific and since they do not give identical results, it is indicated that for a more accurate identification of serotonin in fore-gut carcinoid tumours, a positive reaction with at least two of the applied techniques is desirable.
应用三种不同技术，即嗜银反应、福尔马林诱导的法尔克-希拉尔普荧光法以及使用抗血清素单克隆抗体的免疫细胞化学法，对60例前肠类癌肿瘤进行了血清素含量检测。为评估单个肿瘤细胞的染色荧光情况，将这些方法应用于相同的肿瘤切片。12例肿瘤显示有血清素免疫反应性细胞，其中6例同时为嗜银性。4例肿瘤含有嗜银细胞，但无血清素免疫反应性。使用所有三种技术时，出现了三种类型的肿瘤细胞，即：血清素免疫反应性、非嗜银性且无荧光的细胞；血清素免疫反应性、嗜银性且有荧光的细胞；无血清素免疫反应性、嗜银性且无荧光的细胞。第一种（血清素免疫反应性）细胞类型在肿瘤中最为常见。在一例嗜银细胞数量超过血清素免疫反应性细胞的胃类癌中，发现用改良的沃辛-斯塔里反应显示黑色素呈阳性。由于用于内分泌肿瘤中血清素可视化的技术都并非绝对特异，且它们给出的结果也不相同，因此表明，为更准确地鉴定前肠类癌肿瘤中的血清素，最好至少有两种应用技术呈阳性反应。
