Marshall T, Williams K M
Anal Biochem. 1984 Jun;139(2):502-5. doi: 10.1016/0003-2697(84)90041-1.
The presence of 2-mercaptoethanol in the protein denaturing solution used to prepare samples for high resolution two-dimensional electrophoresis can result in artifacts when the two-dimensional patterns are detected by silver staining. These are (i) spots of discrete isoelectric point and electrophoretic mobility, (ii) horizontal bands of the same electrophoretic mobility as the spots (corresponding predominantly to molecular weights of 54,000 and 68,000), and (iii) vertical streaks of variable length and intensity. Although the nature and source of artifacts have not been positively identified the evidence suggests that they are probably not inherent in the 2-mercaptoethanol itself but rather released by the latter from some component common to both the first- and second-dimension constituents of the electrophoretic system.
在用于制备高分辨率二维电泳样品的蛋白质变性溶液中存在2-巯基乙醇时，用银染法检测二维图谱时会产生假象。这些假象包括：（i）具有离散等电点和电泳迁移率的斑点；（ii）与斑点具有相同电泳迁移率的水平条带（主要对应于分子量为54,000和68,000）；以及（iii）长度和强度可变的垂直条纹。尽管假象的性质和来源尚未得到确切确认，但证据表明它们可能并非2-巯基乙醇本身所固有，而是由后者从电泳系统一维和二维成分共有的某些成分中释放出来的。
