Blaich G, Krell H, Pfaff E
Hoppe Seylers Z Physiol Chem. 1984 Jul;365(7):763-71. doi: 10.1515/bchm2.1984.365.2.763.
Calcium efflux from isolated mitochondria on ruthenium red addition was shown to be biphasic. The rate of efflux from a slowly releasable pool was independent of preincubation. It could be saturated and in extrapolation revealed a maximal rate of 3.6 nmol/(min X mg protein). The efflux from a second, rapidly dischargeable pool was related to calcium added up to 300 nmol/mg protein when a final rate of 15 nmol/(min X mg protein) was reached. The magnitude of the latter pool depended on the time of preincubation in the presence of calcium and correlated with mitochondrial swelling. After ruthenium red addition, a further increase of this pool and spontaneous, destructive calcium release was prevented. Three conclusions are drawn from these results: On preincubation with calcium, part of the mitochondrial calcium develops into a rapidly dischargeable pool. This pool is responsible for mitochondrial alterations resulting in a spontaneous, destructive release of total calcium. Ruthenium red inhibits calcium release by discharging mitochondria from this destructive calcium pool. To avoid artefacts, mitochondrial parameters should be carefully controlled when ruthenium red-insensitive calcium efflux is studied.
添加钌红后，分离出的线粒体中的钙外流呈双相性。来自缓慢释放池的外流速率与预孵育无关。它可以被饱和，外推显示最大速率为3.6 nmol/（分钟×毫克蛋白）。当达到最终速率15 nmol/（分钟×毫克蛋白）时，来自第二个快速释放池的外流与添加的钙有关，添加量可达300 nmol/毫克蛋白。后一个池的大小取决于在钙存在下的预孵育时间，并与线粒体肿胀相关。添加钌红后，该池的进一步增加以及自发的、破坏性的钙释放被阻止。从这些结果得出三个结论：在与钙预孵育时，线粒体钙的一部分发展成快速释放池。这个池负责导致总钙自发、破坏性释放的线粒体改变。钌红通过使线粒体从这个破坏性钙池中释放来抑制钙释放。为避免假象，在研究钌红不敏感的钙外流时，应仔细控制线粒体参数。
