Lehtosalo J I, Uusitalo H, Stjernschantz J, Palkama A
Histochemistry. 1984;80(5):421-7. doi: 10.1007/BF00495429.
The trigeminal ganglion of rat and guinea pig was studied for the presence of immunoreactive substance-P using fluorescence, light and electronmicroscopy. In untreated animals substance P containing cells, with a diameter of 15 to 50 micron, were distributed throughout the ganglion and comprised 10-30% of all ganglion cells. Colchicine, injected intraventricularly to inhibit intra-axonal transport, had no effect on the number of substance P cells; but when the drug was injected directly into the posterior root of the ganglion, the proportion of these cells increased to as much as 50%. In the electron microscope, immunoreactive substance-P was confined to ganglion cells classified as B type according to the arrangement of subcellular organelles, and to unmyelinated nerve fibers. Subcellularly the immunoreactivity appeared in cytoplasmic vesicles, as well as dispersed in the nerve fibers and the perikarya of neurons. The great number of substance P immunoreactive ganglion cells suggests that they do not comprise a well defined subpopulation of the B-cells. However, the immunoreactivity was restricted to a distinct ultrastructural type of neurons with unmyelinated nerve fibers, suggesting that they also may share some distinct functions.
利用荧光显微镜、光学显微镜和电子显微镜，对大鼠和豚鼠的三叉神经节进行了研究，以检测P物质免疫反应性物质的存在情况。在未处理的动物中，直径为15至50微米、含有P物质的细胞分布于整个神经节，占所有神经节细胞的10%至30%。经脑室注射秋水仙碱以抑制轴突内运输，对P物质细胞的数量没有影响；但当将该药物直接注射到神经节的后根时，这些细胞的比例增加到高达50%。在电子显微镜下，P物质免疫反应性物质局限于根据亚细胞器排列归类为B型的神经节细胞以及无髓神经纤维。在亚细胞水平上，免疫反应性出现在细胞质小泡中，也分散在神经纤维和神经元的胞体中。大量具有P物质免疫反应性的神经节细胞表明，它们并不构成B细胞的一个明确界定的亚群。然而，免疫反应性局限于具有无髓神经纤维的一种独特超微结构类型的神经元，这表明它们也可能具有一些独特的共同功能。
