Lehtosalo J I, Uusitalo H, Laakso J, Palkama A, Härkönen M
Histochemistry. 1984;80(3):219-23. doi: 10.1007/BF00495769.
The presence of 5-hydroxytryptamine (5-HT), as well as its precursor (5-HTP) and metabolite (5-HIAA), were biochemically determinated in the trigeminal ganglion of the guinea pig and rat. The distribution of 5-HT in the ganglion and in its posterior root was studied using both indirect immunofluorescence and the peroxidase-antiperoxidase method. In order to increase the possible 5-HT content of primary sensory neurons for subsequent immunohistochemical visualization, animals were first treated with nialamide, an inhibitor of monoamine oxidase, and then loaded with L-tryptophan. Another group of animals received colchicine to inhibit intra-axonal transport of transmitter substances. However, even combined use of loading and colchicine treatment did not reveal 5-HT immunoreactivity in ganglion cells. The only source of 5-HT immunoreactivity in the trigeminal ganglion and its posterior root was mast cells. These cells were located around the ganglion in adjacent leptomeningeal and connective tissues, as well as between the ganglion cells and nerve fibers. Only occasionally were mast cells found in the posterior root of the ganglion.
在豚鼠和大鼠的三叉神经节中，对5-羟色胺（5-HT）及其前体（5-羟色氨酸，5-HTP）和代谢产物（5-羟吲哚乙酸，5-HIAA）进行了生化测定。利用间接免疫荧光法和过氧化物酶-抗过氧化物酶法研究了5-HT在神经节及其后根中的分布。为了增加初级感觉神经元中可能的5-HT含量，以便随后进行免疫组织化学观察，首先用单胺氧化酶抑制剂尼亚酰胺处理动物，然后给予L-色氨酸。另一组动物接受秋水仙碱以抑制递质的轴突内运输。然而，即使联合使用加载和秋水仙碱处理，也未在神经节细胞中显示出5-HT免疫反应性。三叉神经节及其后根中5-HT免疫反应性的唯一来源是肥大细胞。这些细胞位于神经节周围相邻的软脑膜和结缔组织中，以及神经节细胞和神经纤维之间。仅偶尔在神经节后根中发现肥大细胞。
