Sherr D H, Vietor H E, Liu Y N, Dorf M E
J Immunol. 1984 Nov;133(5):2417-22.
The carrier requirements for the induction of helper and suppressor T (Ts) cells were compared. Although H-2-linked Ir genes control the development of helper T cells and hapten-specific B cells, they do not influence Ts3 generation. That is, GL phi nonresponder C57BL/6 mice can generate NP-specific Ts3 cells after priming with NP-GL phi. The Ts3 cells generated under these conditions are functionally and phenotypically identical to the NP-specific Ts3 cells previously characterized. Furthermore, these Ts3 populations can be specifically depleted with a monoclonal anti-idiotope antibody prepared against monoclonal anti-NP antibodies. By using related polymers, carrier effects on Ts3 induction were noted. NP-D-GL and NP-Ficoll failed to induce Ts3 cells, whereas NP-L-GL induced this suppressor subset. The data demonstrate that Ts3 induction is independent of the carrier requirements involved in helper T cell induction and is not dependent upon B cell priming. The implications of these results with regard to the mechanisms of Ts3 induction are discussed.
比较了诱导辅助性T细胞和抑制性T（Ts）细胞的载体需求。虽然与H-2连锁的Ir基因控制辅助性T细胞和半抗原特异性B细胞的发育，但它们不影响Ts3细胞的产生。也就是说，GL phi无反应性的C57BL/6小鼠在用NP-GL phi致敏后可产生NP特异性Ts3细胞。在这些条件下产生的Ts3细胞在功能和表型上与先前鉴定的NP特异性Ts3细胞相同。此外，这些Ts3细胞群体可用针对单克隆抗NP抗体制备的单克隆抗独特型抗体特异性清除。通过使用相关聚合物，观察到载体对Ts3诱导的影响。NP-D-GL和NP-菲可均不能诱导Ts3细胞，而NP-L-GL可诱导该抑制性亚群。数据表明，Ts3诱导独立于辅助性T细胞诱导中涉及的载体需求，且不依赖于B细胞致敏。讨论了这些结果对Ts3诱导机制的意义。
