Hausman P B, Sherr D H, Dorf M E
J Immunol. 1985 Mar;134(3):1388-96.
An in vitro method for the generation of effector suppressor cells (Ts3) was developed. By utilizing this protocol, it was possible to investigate both the cellular and genetic requirements for suppressor cell induction. It was determined that populations containing Ts3 cells can be induced after a 4-day culture of spleen cells and antigen. These Ts3 cells are similar to Ts3 cells generated by in vivo immunization. Both populations are I-J+, bind NP hapten, bind NP hapten, bear receptors which share NPb idiotypic determinants with anti-NP antibodies, function during the effector phase of the immune response, and require activation with Ts2 cells. Generation of Ts3-containing populations required both nylon wool-nonadherent T cells and a nylon-adherent, B cell-enriched population from an Igh-identical donor. T cells cultured with antigen alone or with syngeneic macrophages and antigen did not develop suppressive activity. Lytic treatment of the nylon-adherent population with a B cell-specific monoclonal antibody (J11d) removed the ability to generate suppressor cells. These results imply that the induction of suppressor T cells requires B lymphocytes, and that this induction process is dependent on Igh-linked gene products.
开发了一种体外产生效应抑制细胞（Ts3）的方法。利用该方案，可以研究抑制细胞诱导的细胞和遗传需求。已确定，脾细胞与抗原进行4天培养后，可诱导出含有Ts3细胞的群体。这些Ts3细胞与通过体内免疫产生的Ts3细胞相似。这两种群体均为I-J +，结合NP半抗原，带有与抗NP抗体共享NPb独特型决定簇的受体，在免疫反应的效应阶段发挥作用，并且需要用Ts2细胞激活。产生含Ts3的群体既需要尼龙毛非黏附性T细胞，也需要来自Igh相同供体的尼龙黏附性、富含B细胞的群体。单独用抗原培养T细胞或与同基因巨噬细胞及抗原一起培养T细胞，均不会产生抑制活性。用B细胞特异性单克隆抗体（J11d）对尼龙黏附群体进行裂解处理，消除了产生抑制细胞的能力。这些结果表明，抑制性T细胞的诱导需要B淋巴细胞，并且这种诱导过程依赖于Igh连锁的基因产物。
