Toder V, Nebel L, Gleicher N
J Clin Lab Immunol. 1984 Jul;14(3):123-7.
In using both a 51Cr-release assay and a single cell technique we measured different parameters of the effector lymphocytes killing process in pregnant and control women on a cell population and single cell level. Total NK activity was lower in pregnancy, but the difference was not significant. Parity showed no cumulative effect. Pregnant women had normal percentages of potentially cytotoxic target binding cells, however, the relative number of lymphocytes that could kill bound targets (i.e. active NK cells) was significantly depressed in pregnancy when compared with normal controls (p less than 0.05). This diminished number of active NK lymphocytes was, however, normal in all phases of the lytic procedure. Interferon (IFN) treatment of effector cells in pregnant women in vitro did not alter target cell binding, but did increase the percentage of active NK cells. The level of stimulation by IFN was the same in pregnancy and control patients. The kinetics of response were the same for both cell populations and different doses of interferon caused a similar level of augmentation. In conclusion we suggest that the here demonstrated deficiency of active NK lymphocytes in pregnancy may represent a function of pregnancy associated immunoregulatory molecules which prevent a population of pre-NK cells to express their cytotoxic potential.
通过使用51Cr释放试验和单细胞技术，我们在细胞群体和单细胞水平上测量了孕妇和对照女性中效应淋巴细胞杀伤过程的不同参数。孕期总的自然杀伤（NK）活性较低，但差异不显著。经产情况未显示出累积效应。孕妇中潜在细胞毒性靶细胞结合细胞的百分比正常，然而，与正常对照组相比，孕期中能够杀伤结合靶细胞的淋巴细胞相对数量（即活性NK细胞）显著降低（p<0.05）。然而，活性NK淋巴细胞数量的减少在裂解过程的所有阶段都是正常的。体外对孕妇效应细胞进行干扰素（IFN）处理并未改变靶细胞结合，但确实增加了活性NK细胞的百分比。孕期和对照患者中IFN的刺激水平相同。两个细胞群体的反应动力学相同，不同剂量的干扰素引起相似程度的增强。总之，我们认为此处所证明的孕期活性NK淋巴细胞缺乏可能代表了与妊娠相关的免疫调节分子的一种功能，该功能可阻止一群前NK细胞表达其细胞毒性潜能。
