Katz P, Zaytoun A M, Lee J H, Panush R S, Longley S
J Immunol. 1982 Nov;129(5):1966-71.
The natural killer (NK) cell activity in fifteen systemic lupus erythematosus (SLE) patients was investigated by employing 51-chromium- (51Cr) release microcytotoxicity and single cell cytotoxicity assays against K562 target cells. Although the SLE patients as a group had depressed NK function in the 51Cr-release assay compared to normal subjects (p less than 0.005), those with clinically active disease displayed the greatest impairment in this activity (p less than 0.001). Active SLE patients were deficient in overall NK activity (Vmax) (p less than 0.005) but had normal percentages of potentially cytotoxic target binding cells (TBC). These TBC, however, were unable to normally kill bound target cells (p less than 0.01), which is indicative of a deficiency of "active" NK cells (p less than 0.005). Those NK cells with intact cytotoxic capabilities could "recycle" and repeat the lytic sequence normally. Exposure of normal lymphocytes to SLE sera did not impair any phase of NK function. These studies indicate that defective NK activity in SLE is secondary to an abnormality in the lytic event itself and is not due to a deficiency of NK cells, an abnormality in target binding, or an inability of NK cells to lyse multiple targets. Additionally, serum factors do not appear to play a major etiologic role in the cytotoxic abnormalities of these patients.
采用51铬（51Cr）释放微量细胞毒性试验和针对K562靶细胞的单细胞细胞毒性试验，对15例系统性红斑狼疮（SLE）患者的自然杀伤（NK）细胞活性进行了研究。尽管与正常受试者相比，SLE患者组在51Cr释放试验中的NK功能有所降低（p<0.005），但临床活动期疾病患者的这种活性受损最为严重（p<0.001）。活动期SLE患者的总体NK活性（Vmax）不足（p<0.005），但潜在细胞毒性靶细胞结合细胞（TBC）的百分比正常。然而，这些TBC不能正常杀伤结合的靶细胞（p<0.01），这表明“活性”NK细胞存在缺陷（p<0.005）。那些具有完整细胞毒性能力的NK细胞可以“循环利用”并正常重复裂解序列。将正常淋巴细胞暴露于SLE血清中不会损害NK功能的任何阶段。这些研究表明，SLE患者NK活性缺陷是裂解事件本身异常的继发结果，并非由于NK细胞缺乏、靶细胞结合异常或NK细胞无法裂解多个靶细胞所致。此外，血清因子似乎在这些患者的细胞毒性异常中不起主要病因作用。
