Molinaro G A, Eby W C, Molinaro C A, Bartolomew R M, David G
Mol Immunol. 1984 Sep;21(9):771-4. doi: 10.1016/0161-5890(84)90163-9.
We have tested whether soluble immune complexes obtained by mixing human growth hormone (hGH) with one anti-hGH monoclonal antibody (MAb) can form a precipitin line when diffused against another MAb in a polyethylene glycol containing gel. By testing seven anti-hGH MAbs one against the other in this assay, we have found that 10 pairs of MAbs out of the 21 possible combinations formed a line. Apparently, the first MAb formed soluble hGH dimers that were linked by the second MAb into precipitating linear complexes. Since each precipitin line was formed by the cooperative reaction of two MAbs, this sequential reaction of MAbs may be used in methods for the positive selection of MAbs that are suitable for two-site immunoassays.
我们测试了通过将人生长激素（hGH）与一种抗hGH单克隆抗体（MAb）混合得到的可溶性免疫复合物，在含有聚乙二醇的凝胶中与另一种MAb扩散时是否能形成沉淀线。通过在此试验中对七种抗hGH单克隆抗体相互进行测试，我们发现在21种可能的组合中有10对单克隆抗体形成了沉淀线。显然，第一种单克隆抗体形成了可溶性hGH二聚体，这些二聚体被第二种单克隆抗体连接成沉淀性线性复合物。由于每条沉淀线都是由两种单克隆抗体的协同反应形成的，这种单克隆抗体的顺序反应可用于阳性选择适用于双位点免疫测定的单克隆抗体的方法中。
