Molinaro G A, Bui J D, Eby W C
Department of Microbiology, Loma Linda University, CA 92350.
Int J Clin Lab Res. 1992;21(3):235-40. doi: 10.1007/BF02591653.
We have studied the epitopes of the human IgA molecule by using 27 monoclonal antibodies in two simple gel diffusion methods. By testing pairs of monoclonal antibodies for coprecipitation of an IgA myeloma protein, we have clearly identified sterically independent epitopes on the molecule. By testing the non-coprecipitating monoclonal antibodies with soluble IgA-monoclonal antibody immune complexes, we have identified the overlapping epitopes. Eleven proto-epitopes were mapped on the IgA molecule. Since we have not used solid-phase methods, we have presumably identified native epitopes of the IgA molecule.
我们通过两种简单的凝胶扩散方法，使用27种单克隆抗体研究了人IgA分子的表位。通过检测成对单克隆抗体对IgA骨髓瘤蛋白的共沉淀情况，我们明确鉴定出该分子上空间独立的表位。通过用可溶性IgA-单克隆抗体免疫复合物检测不发生共沉淀的单克隆抗体，我们鉴定出了重叠表位。在IgA分子上绘制了11个原表位。由于我们未使用固相方法，推测我们鉴定出的是IgA分子的天然表位。
