Elseweidy M, Singh M
Proc Soc Exp Biol Med. 1984 Nov;177(2):247-52. doi: 10.3181/00379727-177-41938.
The present study was designed to determine the effect of folate deficiency on pancreatic acinar cell function. In the first series of experiments, three groups of rats were fed ad libitum regular rat feed, folate-deficient diet, or an equivalent amount of folate-sufficient diet. In the second series of experiments, rats were either fed ad libitum or rendered folate deficient by a purified folate-deficient diet; half of the folate-deficient group was replenished with oral folate. Body weight, pancreatic weight, DNA [methyl-14C]thymidine incorporation into DNA, RNA, [8-14C]adenine incorporation into RNA, protein content, synthesis of proteins, amylase content, and basal and bethanechol-stimulated amylase secretion were determined. The parameters were the same in the rats fed a folate-sufficient diet as in those fed a regular rat feed. Feeding a folate-deficient diet resulted in impaired DNA synthesis as evidenced by diminished incorporation of [methyl-14C]thymidine into DNA. There was no change in secretion of amylase. Similar results were obtained in the second series of experiments. These studies indicate that folate deficiency (rather than antibiotic content of the diet) impaired pancreatic function. Folate deficiency may therefore contribute to pancreatic injury in malnutrition and alcoholism.
本研究旨在确定叶酸缺乏对胰腺腺泡细胞功能的影响。在第一组实验中，三组大鼠随意进食常规大鼠饲料、叶酸缺乏饮食或等量的叶酸充足饮食。在第二组实验中，大鼠要么随意进食，要么通过纯化的叶酸缺乏饮食使其叶酸缺乏；叶酸缺乏组的一半用口服叶酸补充。测定了体重、胰腺重量、DNA中[甲基-14C]胸苷掺入DNA、RNA中[8-14C]腺嘌呤掺入RNA、蛋白质含量、蛋白质合成、淀粉酶含量以及基础和氨甲酰甲胆碱刺激的淀粉酶分泌。喂食叶酸充足饮食的大鼠的参数与喂食常规大鼠饲料的大鼠相同。喂食叶酸缺乏饮食导致DNA合成受损，这可通过[甲基-14C]胸苷掺入DNA减少来证明。淀粉酶分泌没有变化。在第二组实验中获得了类似的结果。这些研究表明，叶酸缺乏（而非饮食中的抗生素含量）损害了胰腺功能。因此，叶酸缺乏可能导致营养不良和酒精中毒中的胰腺损伤。
