Menczel E, Bucks D, Maibach H, Wester R
Arch Dermatol Res. 1984;276(5):326-9. doi: 10.1007/BF00404626.
Human autopsy skin was sliced into three sections; an outer epidermis-rich layer, a middle dermis layer, and an inner dermis and subcutaneous fat layer. Each slice was bathed in lindane solutions over a 50-fold concentration range for 48 h at 37 degrees C. Lindane uptake by the skin was extensive (less than 90%) for all concentrations and all skin slices. Equilibrium dialysis with lindane at 37 degrees C for 24 h showed binding affinities by the skin sections to exceed 98%. The binding constants of the outer skin section (epidermis-rich) were higher than those of the middle and inner sections. The binding attraction for lindane by United States Pharmacopeia (USP) human plasma protein fraction (mean 90.5% bound) resulted in its competitive extraction from the skin slices in a three-compartment dialytic procedure. Since the least binding constants were in the middle and inner layers, and since lindane has a preference for plasma over skin, the two (inner skin and plasma) may combine to create the sink conditions necessary for lindane percutaneous absorption into the body. This plus the high epidermis capacity for lindane explains why in some instances (such as occlusion) high levels of lindane can be percutaneously absorbed.
外层富含表皮的层、中间的真皮层以及内层的真皮和皮下脂肪层。将每片皮肤在浓度范围为50倍的林丹溶液中于37摄氏度下浸泡48小时。对于所有浓度和所有皮肤切片，林丹在皮肤中的摄取量都很高（小于90%）。在37摄氏度下用林丹进行24小时的平衡透析表明，皮肤切片的结合亲和力超过98%。外层皮肤切片（富含表皮）的结合常数高于中间层和内层。美国药典（USP）人血浆蛋白部分对林丹的结合吸引力（平均90.5%结合）导致在三室透析过程中林丹从皮肤切片中被竞争性提取出来。由于最低的结合常数存在于中间层和内层，并且由于林丹对血浆的亲和力高于皮肤，这两者（内层皮肤和血浆）可能共同作用，创造出林丹经皮吸收进入体内所需的汇流条件。这一点加上表皮对林丹的高容纳能力，解释了为什么在某些情况下（如封闭状态）高水平的林丹能够被经皮吸收。
