[Specificity of the enzymes of Salmonella O-antigen biosynthesis. 6. Synthesis of sugar nucleotides with glycosyl phosphate activation. Analogs of guanosine diphosphate mannose modified at position 6 ofthe purine ring].

Interaction of alpha-D-mannopyranosyl phosphate with diphenyl phosphochloridate gave the trisubstituted pyrophosphate which was converted through the reaction with nucleoside 5'-phosphates into nucleoside 5'-(alpha-D-mannopyranosyl)pyrophosphates. The method was used for preparation of guanosine diphosphate mannose analogs derived from adenine, purine, 2-aminopurine, 2-amino-6-methoxypurine, 2-amino-6-chloropurine, and 2-amino-6-mercaptopurine. These analogs are necessary for study on substrate specificity of mannosyltransferases of Salmonella O-specific polysaccharides biosynthesis.